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The O2 is exceptionally well-served by public transport, with the Jubilee Line and Docklands Light Railway (DLR) providing direct access. The nearest station is North Greenwich, which is directly adjacent to The O2 complex. This station is served by the Jubilee Line, offering a quick and direct connection to key London hubs such as Canary Wharf, London Bridge, Westminster, and Bond Street. The DLR also provides a convenient link, particularly for those travelling from areas in East and South London. Trains from major UK cities like London, Manchester, Birmingham, and Edinburgh will typically arrive at major London terminals such as London Euston, St Pancras International, or King's Cross. From these stations, a short journey on the Underground (Jubilee Line for Euston and St Pancras, or interchange via Victoria for King's Cross) will bring you directly to North Greenwich. Journey times from central London stations to North Greenwich are usually under 20 minutes. North Greenwich station offers standard facilities including ticket machines and accessible routes.

Numerous bus routes serve The O2, making it easily accessible from across London. The primary bus stop is located directly outside the main entrance at North Greenwich. Key bus routes that stop here include the 108, which runs 24/7 between Lewisham and Stratford, providing a vital link through South East London. Other important routes include the 132 from Bexleyheath to Greenwich, the 286 from Sidcup to Greenwich, and the 422 from Bexleyheath to North Greenwich. These routes connect The O2 to a wide range of residential areas and town centres across South East London and parts of Kent. For those travelling from further afield or during late hours, the 108 is a crucial night bus service. Journey times from central London locations like Oxford Circus or Piccadilly Circus can range from 30 to 50 minutes depending on traffic conditions. Bus services are operated by Transport for London (TfL) and its contracted operators.

Driving to The O2 is straightforward, with clear signposting from major routes. If approaching from the M25, exit at Junction 2 and follow signs for the A2 towards Central London. Continue on the A2, then follow signs for the A102 (Blackwall Tunnel). After passing through the Blackwall Tunnel, keep left and follow signs for The O2/North Greenwich. The postcode for sat-nav users is SE10 0DX. The O2 has its own multi-storey car park, operated by Q-Park, offering ample parking for visitors. Additional parking is often available in surrounding areas, though this can be more limited and expensive. Parking costs at The O2's official car park vary depending on the duration and whether it's pre-booked, but typically range from approximately £8-£30 for a standard event visit. There are designated accessible parking bays available for blue badge holders within the main car park. No official park and ride service operates directly to The O2, but onward travel from park and ride facilities on the outskirts of London is possible via public transport.

The O2 is committed to providing an accessible experience for all visitors. Wheelchair access is available throughout the venue, including all entrances, concourses, and public areas, with lifts providing access to different levels. Dedicated accessible seating areas are available for most events, offering excellent views of the stage or pitch. Accessible toilets are clearly signposted and available on all levels. The venue is equipped with an induction loop system to assist visitors with hearing impairments; this can usually be accessed by switching hearing aids to the 'T' setting. Assistance dogs and service animals are welcome at The O2; designated relief areas are available. While there is no dedicated accessible parking specifically for The O2, the Q-Park car park offers a number of disabled bays located on lower levels for easier access. Venue staff are trained to provide assistance to visitors who may need help navigating the venue or require support during their visit.