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The primary train station serving The O2 is North Greenwich Underground station, which is conveniently located adjacent to the venue. This station is served by the Jubilee Line, offering direct connections to central London locations such as Canary Wharf, London Bridge, Waterloo, and Bond Street. From major UK cities, you would typically travel into London via one of the capital's main termini (e.g., King's Cross, Euston, Liverpool Street) and then connect to the Jubilee Line. For example, a train from Manchester Piccadilly to London Euston takes approximately 2.5 hours, followed by a short Jubilee Line journey to North Greenwich. Trains to London run frequently throughout the day from all major cities. North Greenwich station is equipped with ticket machines and provides step-free access, enhancing its usability for all passengers.

Numerous bus routes serve the North Greenwich area, providing excellent connectivity to The O2 from across London. Key bus routes include the 108, which runs 24/7 between Lewisham and Stratford via North Greenwich, and the 129 connecting to Greenwich town centre and Plaistow. Other useful routes include the 132 (to Bexleyheath), 161 (to Chingford), 180 (to Catford), 188 (to Bloomsbury via the Rotherhithe Tunnel), and 422 (to Bexleyheath via Woolwich). The majority of these services stop directly at the North Greenwich bus station, immediately outside the arena. Night bus services, such as the N108 and N188, ensure you can get home even after late-night events. Journey times from central London can vary depending on traffic, typically ranging from 30 to 60 minutes.

For those travelling by car, The O2 is easily accessible via the A102, which connects to the M25 (Junction 2) and Blackwall Tunnel. The postcode for sat-nav systems is SE10 0DX. The venue has its own multi-storey car park, offering extensive spaces. Parking is usually charged per hour or as a day rate, with prices varying depending on the event and time of year. It is advisable to book parking in advance online, especially for popular events, to secure a space and potentially a better rate. While there are no dedicated park and ride facilities directly linked to The O2, several TfL car parks are available at Tube stations further out on the Jubilee Line, from which you can travel to North Greenwich. Accessible parking bays are available within the venue's car park for blue badge holders.

The O2 Arena is committed to providing an accessible experience for all its visitors. The venue is largely wheelchair accessible, with accessible entrances, lifts to all public areas, and dedicated wheelchair viewing spaces in the arena bowl. Accessible toilet facilities are available throughout the venue, and all are clearly signposted. Hearing assistance is provided via an induction loop system within the arena; visitors should check with venue staff upon arrival for specific locations. Assistance dogs are welcome, and facilities for service animals are available. Accessible parking is located within the main car park, and staff are trained to offer assistance to visitors with disabilities, ensuring a smooth and enjoyable visit. Visitors are encouraged to contact the venue's accessibility team in advance to discuss specific requirements.